Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10881812 | Cell Biology International | 2005 | 8 Pages |
Abstract
We tested the hypothesis that usual exercise oxidative stress strongly affects erythrocytes viability. A 120-min physical exercise with progressive intensity was used as a model of oxidative stress. FT-IR spectrometry was used to determine structural changes in erythrocyte contents (phospholipids, proteins, lactate, and glucose) from blood samples taken every 20Â min. Carbonyl formation from amino acid residues (PÂ =Â 0.03) and hemoglobin unfolding (PÂ =Â 0.01) could be identified as main protein denaturation markers during oxidative stress. Higher unsaturation level (PÂ =Â 0.001) in phospholipids fatty acyl chains were also observed while VO2 increased (PÂ <Â 0.05). The increase in lactacidosis affected primarily hemoglobin unfolding (PÂ =Â 0.02). Finally, two distinct cellular events occurred during oxidative stress: 1 - phospholipids peroxidation correlated to VO2, but lactacidosis and hemoconcentration remained secondary factors; 2 - hemoglobin denaturation was mainly observed through unfolding and carbonylation, and lactacidosis and hemoconcentration were important contributing factors.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Cyril Petibois, Gérard Déléris,