Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10881815 | Cell Biology International | 2005 | 7 Pages |
Abstract
The Rho-LIM-kinase (LIMK) signaling pathway, believed to be involved in the regulation of tumor invasion, specifically regulates the activity of cofilin. However, it is unclear whether cofilin plays a pivotal role in tumor invasiveness. In this paper we show using a tet-on gene expression system that over-expression of cofilin inhibits the invasiveness of human lung cancer H1299 cells. Over-expressed cofilin disrupts the actin cytoskeleton at the leading edge of the cell and up-regulates p27kip1, which is known to be involved in regulating cell motility. Removal of cofilin over-expression normalizes the p27kip1 level and concomitantly restores the invasiveness of the cultured cells. These findings suggest that excessive cofilin production might prevent cancer cell invasion.
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Authors
Yi-Jang Lee, Dawn J. Mazzatti, Zhong Yun, Peter C. Keng,