Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10881826 | Cell Biology International | 2005 | 7 Pages |
Abstract
The extracellular matrix of the jellyfish Aurelia aurita (Scyphozoa, Cnidaria), known as the mesoglea, is populated by numerous mesogleal cells (Mc). We determined the pattern of the Mc and the mesoglea, raised polyclonal antibodies (RA47) against the major mesogleal protein pA47 (47Â kDa) and checked their specificity. In the mesoglea, RA47 stains pA47 itself. In immunoblots of Mc, RA47 stains bands of 120Â kDa and 80Â kDa; weaker staining is observed at pA47. The same staining pattern is seen on blots of jellyfish epidermal cells and of whole Hydra (Hydrozoa) or isolated mesoglea of Hydra. Our data indicate that pA47 is synthesized by Mc and epidermal cells as high molecular precursors. Using immunostaining techniques, we showed Mc to be involved in the formation of mesogleal non-collagenous (called “elastic” in classic morphological studies) fibres. The biochemical and morphological data suggest that Mc originate from the epidermis.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Tatiana Shaposhnikova, Ivan Matveev, Tatiana Napara, Olga Podgornaya,