| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10881905 | Cell Biology International | 2005 | 5 Pages |
Abstract
A method of Agrobacterium-mediated genetic transformation of sugarbeet (Beta vulgaris L.) with vacuum infiltration has been developed. Aseptic 3-weeks old etiolated seedlings of two diploid O-type sugarbeet lines (KS3 and KS7) have been used for genetic transformation. Transgenic sugarbeet plants carrying the reporter β-glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. Integration of transgenes into sugarbeet genome was confirmed with GUS assay and PCR using primers for bar and gusA genes.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
E.M. Kishchenko, I.K. Komarnitskii, N.V. Kuchuk,