| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10881906 | Cell Biology International | 2005 | 7 Pages |
Abstract
An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.
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Authors
I.O. Andreev, K.V. Spiridonova, V.T. Solovyan, V.A. Kunakh,