Article ID Journal Published Year Pages File Type
10881908 Cell Biology International 2005 7 Pages PDF
Abstract
We studied CH2 and CH3 resonances in cultured cell lines treated with etoposide and fludarabine or bioflavonoid quercetin. Etoposide treatment (10 μM, 18 h) resulted in 3.3 fold increase of the CH2/CH3 signal intensity ratio and 6.4 fold decrease in choline signal of MT4 cells. Incubation of Namalwa cells with fludarabine (3 μM, 72 h) increased the CH2/CH3 signal intensity ratio by 2.4 fold and choline resonance intensity was unchanged. Quercetin treatment (30 μM, 1.5 month) increased CH2/CH3 ratio by 2.1 fold. Necrotic cell death upon ethanol (20%) or DMSO (30%) treatment did not change the CH2/CH3 signal intensity ratio. 1H NMR-based study of mobile lipid domains is sensitive for detection of early engagement into apoptosis.
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