Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10882037 | Cell Biology International | 2005 | 9 Pages |
Abstract
Embryoid bodies, which are similar to post-implantation egg-cylinder stage embryos, provide a model for the study of embryo development and stem cell differentiation. We describe here a novel method for generating embryoid bodies from murine embryonic stem (ES) cells cultured on the STO feeder layer. The ES cells grew into compact aggregates in the first 3 days of coculture, then became simple embryoid bodies (EBs) possessing primitive endoderm on the outer layer. They finally turned into cystic embryoid bodies after being transferred to Petri dishes for 1-3 days. Evaluation of the EBs in terms of morphology and differentiating potential indicates that they were typical in structure and could generate cells derived from the three germ layers. The results show that embryoid bodies can form not only in suspension culture but also directly from ES cells cultured on the STO feeder layer.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Qing-Jun Zhou, Jian-Zhong Shao, Li-Xin Xiang, Ruo-Zhen Hu, Yong-Liang Lu, Hang Yao, Li-Cheng Dai,