Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10883066 | Mitochondrion | 2013 | 5 Pages |
Abstract
A mitochondrial matrix-specific p53 construct (termed p53-290) in HepG2 cells was utilized to determine the impact of p53 in the mitochondrial matrix following oxidative stress. H2O2 exposure reduced cellular proliferation similarly in both p53-290 and vector cells, and p53-290 cells demonstrating decreased cell viability at 1Â mM H2O2 (~Â 85% viable). Mitochondrial DNA (mtDNA) abundance was decreased in a dose-dependent manner in p53-290 cells while no change was observed in vector cells. Oximetric analysis revealed reduced maximal respiration and reserve capacity in p53-290 cells. Our results demonstrate that mitochondrial matrix p53 sensitizes cells to oxidative stress by reducing mtDNA abundance and mitochondrial function.
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Authors
Christopher A. Koczor, Rebecca A. Torres, Earl J. Fields, Amy Boyd, William Lewis,