Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10883622 | Progress in Biophysics and Molecular Biology | 2015 | 8 Pages |
Abstract
Single-molecule fluorescence imaging with living cells offers a new approach to visualize and monitor individual proteins during their cellular activities. It facilities the study of cell signaling proteins whose structures and interactions are highly temporal and spatial regulated. In this review, we will mainly present our recent work on probing the dynamics of two types of important transmembrane growth factor receptors, transforming growth factor β receptors and epidermal growth factor receptors, by single-molecule fluorescence microscopy. This includes the characterization of receptor stoichiometry, monomer-dimer interconversion kinetics, effects of microenvironment on receptor membrane diffusion, and intracellular transportation under different signaling conditions. Related studies on these receptors from other groups, as well as the method developments, will be also discussed. Single-molecule study helps to achieve a better understanding of the molecular mechanism of receptor activation, endocytosis and other molecular events in transmembrane signaling.
Keywords
HMMUAAType III TGFβ receptorSSIMTIRFMTβRITβRIIEGFRTGFHER2HER3TCVUnnatural amino acidtransforming growth factortransforming growth factor β receptorSingle-molecule fluorescence imagingDynamicsSpeedstormSTEDHidden Markov modelstochastic optical reconstruction microscopystimulated emission depletion microscopyTotal internal reflection fluorescence microscopyPhotoactivated localization microscopyPalmSignal transductionEpidermal growth factor receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biophysics
Authors
Yahong Sun, Nan Li, Xiaohong Fang,