| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889302 | Journal of Immunological Methods | 2009 | 6 Pages |
Abstract
We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4Â h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2Â h IP. We further show that the protocol readily quantified histone modifications in chromatin from 104 Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.
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Authors
Michael L. Sikes, Justin M. Bradshaw, Wendell T. Ivory, Jessica L. Lunsford, Ruth E. McMillan, Clayton R. Morrison,