| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889343 | Journal of Immunological Methods | 2008 | 9 Pages |
Abstract
Taken together our results show that we were able to successfully isolate and characterize recombinant scFvs that differentially recognize diverse denatured forms or assembly intermediates of the heat-labile enterotoxin B subunit of E. coli.
Keywords
E. coliEtxBsingle-chain Fv antibody fragmentscFvsETXCTXGM1scFvDTTCDRPBSmAbMBPMonoclonal antibodyRecombinant antibodiesEscherichia coliSDS-PAGEsodium dodecylsulphate polyacrylamide gel electrophoresisheat-labile enterotoxinEpitopeEnzyme-linked immunosorbent assayELISAdithiothreitolcholera toxinphosphate buffer salinecomplementary determining regionProtein assemblyPhage displayMyelin basic protein
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Authors
Wen Yuan Chung, Markus Sack, Rachel Carter, Holger Spiegel, Rainer Fischer, Timothy R. Hirst, Neil A. Williams, Roger F.L. James,