Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889346 | Journal of Immunological Methods | 2008 | 5 Pages |
Abstract
This study evaluated the usefulness of testing sera with perinuclear antineutrophil cytoplasmic antibodies (P-ANCA) on formalin-fixed neutrophils to differentiate between vasculitis and other diseases, and to distinguish between myeloperoxidase (MPO) and other antigen targets. Sera from active (n = 24) or treated vasculitis (n = 23) and non-vasculitic disease (n = 37) were tested in 3 ethanol- and 2 formalin-fixed neutrophil assays, and in 12 MPO-ANCA ELISAs. The sensitivity of demonstrating that P-ANCA became cytoplasmic on formalin-fixed cells in vasculitis, and negative in non-vasculitic disease was 75-79% in different assays compared with 88% for positivity in the majority of MPO-ANCA ELISAs. The sensitivity and specificity of demonstrating that P-ANCA became cytoplasmic where the target was MPO, and disappeared with other antigens were 89% and 74-80% respectively in different assays. P-ANCA specificity for MPO should be confirmed in one of the better-performing MPO-ANCA ELISAs.
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Authors
Wendy Pollock, Michelle Trevisin, Judy Savige, the Australasian ANCA Study Group the Australasian ANCA Study Group,