Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889349 | Journal of Immunological Methods | 2008 | 10 Pages |
Abstract
In summary, we have measured immune responsiveness by using repeated immunizations with influenza virus vaccine as the model antigen. The induction of comparable B-cell responses after the second and third serial immunizations provides a possibility to investigate effects on immune responsiveness by immunomodulatory drugs. The method also allows humoral memory and immune competence per se to be studied on a cellular level in different patient groups.
Keywords
fluorescent-activated cell sorterFACSTNFB-cell ElispotPBMCenzyme linked immunospot assayFasciaELISPOTBSAbovine serum albuminantibodiesenzyme linked immunosorbent assayimmunoglobulin interferonIFNinterleukinELISAB-cellsperipheral blood mononuclear cellsCoefficient of Variationtumor necrosis factorImmune monitoringVaccinationImmune response
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Authors
Therese Vallerskog, Hans Gaines, Adina Feldman, Eric Culbert, Lars Klareskog, Vivianne Malmström, Christina Trollmo,