Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889377 | Journal of Immunological Methods | 2005 | 13 Pages |
Abstract
We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex® BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex® BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/μl and compared with samples collected in K3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex® BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex® BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.
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Authors
Dominic E. Warrino, Louis J. DeGennaro, Mary Hanson, Susan Swindells, Samuel J. Pirruccello, Wayne L. Ryan,