Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10890959 | Microbiological Research | 2005 | 5 Pages |
Abstract
Considering that sensitive and specific methods to detect HSV-1, CMV and HHV-6 on oral mucosa have a great impact on oral diagnosis practice and research, together with the evidence that PCR is a rapid and reliable method, the purpose of the present study was to develop primer sets to detect HSV-1, CMV and HHV-6 in oral swabs by nested polymerase chain reaction (nested PCR). We developed a practical method for sample collection without tissue trauma, and the swabs were stored until used for DNA extraction. After the nested PCR a DNA fragment of 241Â bp corresponding to HSV-1 was amplified. DNA fragments of 224 and 369Â bp were amplified corresponding to CMV and HHV-6, respectively. DNA sequencing analysis confirmed the expected sequences of each virus. In conclusion, it was demonstrated that these new primer sets are able to identify HSV-1, CMV and HHV-6 in oral swab using nested PCR.
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Authors
Júnia Maria Netto Victória, André Luiz Sena Guimarães, Luciano Marques da Silva, Evanguedes Kalapothakis, Ricardo Santiago Gomez,