Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10899685 | Cancer Letters | 2015 | 5 Pages |
Abstract
The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.
Keywords
FITCTSH-receptorTTF1fluoroisothiocyanateDTCEpCAMTPOTSHStgqRT-PCRCECGAPDHmRNACTCcDNANiScomplementary deoxyribonucleic acidDifferentiated thyroid cancerThyroperoxidasemessenger ribonucleic acidepithelial cell adhesion moleculethyroid stimulating hormonequantitative real-time PCRcirculating tumour cellsglyceraldehyde 3-phosphate dehydrogenase
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Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Sarah Sorg, Katharina Pachmann, Katya Brede-Hekimian, Martin Freesmeyer, Thomas Winkens,