Identification of the factors responsible for the in vitro pro-oxidant and cytotoxic activities of the olive polyphenols oleuropein and hydroxytyrosol

Article ID	Journal	Published Year	Pages	File Type
10900025	Cancer Letters	2013	9 Pages	PDF

Abstract

âº The in vitro effects of olive polyphenols oleuropein and hydroxytyrosol are due to H2O2 production. âº H2O2 causes oxidative DNA damage and a decrease in cell viability of cancer and normal cells alike. âº Sodium bicarbonate is essential for the production of H2O2 by olive polyphenols. âº Our results challenge previous data interpretations of in vitro studies with olive polyphenols.

Keywords

FOX FBS EBSS 8-oxo-dG NAC NaHCO3 10-Acetyl-3,7-dihydroxyphenoxazine 7,8-dihydro-8-oxo-2′-deoxyguanosine DMEM/F12 Hydrogen peroxide Oxidative stress minimal essential medium Earle’s Balanced Salt Solution sodium bicarbonate fetal bovine serum MEM N-acetyl cysteine H2O2 Pro-oxidants