Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10900328 | Cancer Letters | 2005 | 7 Pages |
Abstract
The Ru(III) complex salt KP1019 induced formation of H2O2 in colorectal tumor cells in a dose-dependent way. It also caused DNA-strand breaks if only weakly doubling tail length to 55.87±3.97 μm. Both effects were prevented by 5 mM N-acetylcysteine (NAC) which also reduced cytotoxicity (IC50 55 vs 30 μM without NAC). Induction of apoptosis was shown by loss of mitochondrial membrane potential in 63.4±2.1% of the population and by caspase-dependent cleavage of poly-(ADP-ribose)-polymerase (PARP). Both effects were inhibited by NAC which reduced the population with depolarized mitochondrial membranes to 24.1±1.2% and prevented PARP-cleavage indicating a central role oxidative stress in KP1019-induced apoptosis.
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Authors
Susanne Kapitza, Michael A. Jakupec, Maria Uhl, Bernhard K. Keppler, Brigitte Marian,