Article ID Journal Published Year Pages File Type
10900404 Cancer Letters 2005 14 Pages PDF
Abstract
To explore whether DNA polymerase beta (pol β) contributes to the malignant transformation of gastric mucosa, we examined pol β in gastric tumor cell lines, primary tumors and precancerous lesions. Point mutations of pol β were detected in 6 of 13 cell lines and 23 of 104 tissues including 35.0% (14/40) of gastric cancer (GC), 30.0% (3/10) of dysplasia (Dys), 28.6% (4/14) of intestinal metaplasia (IM) and 10.5% (2/19) of chronic atrophic gastritis (CAG), respectively. A frequent mutation was a T to C transition at nucleotide 889, which was observed in 4 GC cell lines, 7 GC, 2 Dys, and 2 IM. The level of pol β expression in tumors was higher than that of their matched normal tissues and gradual changes from GC, Dys, CAG to IM. These results indicate that the mutation and overexpression of pol β may influence the progression during gastric carcinogenesis.
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Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research
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