Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10903703 | Experimental Cell Research | 2016 | 11 Pages |
Abstract
Benign prostatic hyperplasia (BPH) is one of the major disorders of the urinary system in elderly men. Docosahexaenoic acid (DHA) is the main component of n-3 polyunsaturated fatty acids (n-3 PUFAs) and has nerve protective, anti-inflammatory and tumour-growth inhibitory effects. Here, the therapeutic potential of DHA in treating BPH was investigated. Seal oil effectively prevented the development of prostatic hyperplasia induced by oestradiol/testosterone in a rat model by suppressing the increase of the prostatic index (PI), reducing the thickness of the peri-glandular smooth muscle layer, inhibiting the proliferation of both prostate epithelial and stromal cells, and downregulating the expression of androgen receptor (AR) and oestrogen receptor α (ERα). An in vitro study showed that DHA inhibited the growth of the human prostate stromal cell line WPMY-1 and the epithelial cell line RWPE-1 in a dose- and time-dependent manner. In both cell lines, the DHA arrested the cell cycle in the G2/M phase. In addition, DHA also reduced the expression of ERα and AR in the WPMY-1 and RWPE-1 cells. These results indicate that DHA inhibits the multiplication of prostate stromal and epithelial cells through a mechanism that may involve cell cycle arrest and the downregulation of ERα and AR expression.
Keywords
PCNACCNB1α-SMACyclin B1SMCGAPDHCCND1HprtGPR30ERαERβCyclin D1Proliferating Cell Nuclear Antigendocosahexaenoic acidα-smooth muscle actinDHASmooth muscle cellBenign prostatic hyperplasiaBPHHypoxanthine phosphoribosyltransferase 1Cell cycleglyceraldehyde-3-phosphate dehydrogenaseAndrogen ReceptorOestrogen receptor αG protein-coupled receptor 30
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Authors
Chao Wang, Fei Luo, Ying Zhou, Xiaoling Du, Jiandang Shi, Xiaoling Zhao, Yong Xu, Yan Zhu, Wei Hong, Ju Zhang,