Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10904034 | Experimental Cell Research | 2014 | 12 Pages |
Abstract
GPNMB/OA contribution in PCa malignant phenotype has been analyzed by small interfering RNA-induced GPNMB/OA silencing. We found that following GPNMB/OA silencing the migration capability of both DU145 and PC3 cells, evaluated by using in vitro invasivity assay, as well as the metalloproteinases MMP-2 and MMP-9 activity were equally strongly inhibited. By contrast knocking down GPNMB/OA weakly attenuated cell proliferation rate of DU145, an effect that paralleled with an increase number of apoptotic cells. However, PC3 cell growth seems to be not affected by GPNMB/OA. Together, these data reveal that GPNMB/OA acts as a critical molecular mediator promoting the acquisition of the more aggressive, pro-metastatic phenotype distinctive of human DU145 and PC3 cell lines.
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Authors
Chiara Fiorentini, Serena Bodei, Francesca Bedussi, Martina Fragni, Sara Anna Bonini, Claudio Simeone, Danilo Zani, Alfredo Berruti, Cristina Missale, Maurizio Memo, PierFranco Spano, Sandra Sigala,