Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10904260 | Experimental Cell Research | 2013 | 10 Pages |
Abstract
Methionine adenosyltransferase (MAT) is an essential enzyme that is responsible for the biosynthesis of S-adenosylmethionine (SAMe), the principal methyl donor and precursor of polyamines. MAT1A is expressed in normal liver and MAT2A is expressed in all extrahepatic tissues. MAT2A expression is increased in human colon cancer and in colon cancer cells treated with mitogens, whereas silencing MAT2A resulted in apoptosis. The aim of the current work was to examine the mechanism responsible for MAT2A-dependent growth and apoptosis. We found that in RKO (human adenocarcinoma cell line) cells, MAT2A siRNA treatment lowered cellular SAMe and putrescine levels by 70-75%, increased apoptosis and inhibited growth. Putrescine supplementation blunted significantly MAT2A siRNA-induced apoptosis and growth suppression. Putrescine treatment (100Â pmol/L) raised MAT2A mRNA level to 4.3-fold of control, increased the expression of c-Jun and c-Fos and binding to an AP-1 site in the human MAT2A promoter and the promoter activity. In human colon cancer specimens, the expression levels of MAT2A, ornithine decarboxylase (ODC), c-Jun and c-Fos are all elevated as compared to adjacent non-tumorous tissues. Overexpression of ODC in RKO cells also raised MAT2A mRNA level and MAT2A promoter activity. ODC and MAT2A are also overexpressed in liver cancer and consistently, similar MAT2A-ODC-putrescine interactions and effects on growth and apoptosis were observed in HepG2 cells. In conclusion, there is a crosstalk between polyamines and MAT2A. Increased MAT2A expression provides more SAMe for polyamines biosynthesis; increased polyamine (putrescine in this case) can activate MAT2A at the transcriptional level. This along with increased ODC expression in cancer all feed forward to further enhance the proliferative capacity of the cancer cell.
Keywords
scrambled siRNAAP-1EGFIGF-1Ornithine decarboxylaseODC5-bromo-2′-deoxyuridineHCCSmall interfering RNAsiRNAS-adenosylmethionineinsulin-like growth factor-1chromatin immunoprecipitationBrdULiver cancerColon cancerepidermal growth factorputrescineMatmethionine adenosyltransferasepolymerase chain reactionPCRactivator protein-1CHiPHepatocellular carcinomahigh-performance liquid chromatographyHPLCSAMe
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Maria Lauda Tomasi, Minjung Ryoo, Anna Skay, Ivan Tomasi, Pasquale Giordano, José M. Mato, Shelly C. Lu,