| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10904333 | Experimental Cell Research | 2013 | 9 Pages |
Abstract
⺠Primary and immortalized endothelial cultures were established from mice capillaries. ⺠Monoclonal cell lines were obtained and compared to primary cultured cells. ⺠Monoclonal cell lines retained morphological characteristics and functional properties. ⺠This endothelial cell line can be useful in the field of microvascular research.
Keywords
FACSPLA2eNOSDAPIVCAM-1vWFR-PhycoerythrinACEPDTPGI2FITCDAF-2 DAFBSUEA-1DMEM4′,6-diamidino-2-phenylindole4,5-diaminofluorescein diacetateDMSODulbecco's modified Eagle's mediumangiotensin converting enzymephospholipase A2AChbradykininfluorescence-activated cell sortingDimethylsulfoxideMicrocirculationPopulation doubling timefetal bovine serumEndothelial cellendothelial nitric oxide synthaseVon Willebrand factorfluorescein isothiocyanatevascular cell adhesion moleculeNitric oxideprostacyclin
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Authors
Rodrigo A. Loiola, Tathiany C. Torres, Carla M. Aburaya, Maristella A. Landgraf, Richardt G. Landgraf, João Bosco Pesquero, Liliam Fernandes,