| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10904355 | Experimental Cell Research | 2013 | 12 Pages |
Abstract
⺠Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ⺠Tri-molecular complexes containing two scFvs and one molecule GK were formed. ⺠GK was detected using fluorescence lifetime-based FRET imaging. ⺠GK is monomeric and homogeneously distributed in several cancer cell lines. ⺠This technique may have many applications in live-cell imaging of endogenous proteins.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Anne-Sophie Rinaldi, Guillaume Freund, Dominique Desplancq, Annie-Paule Sibler, Mireille Baltzinger, Natacha Rochel, Yves Mély, Pascal Didier, Etienne Weiss,