Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10904503 | Experimental Cell Research | 2011 | 14 Pages |
Abstract
Factor for adipocyte differentiation 104 (fad104) is a regulator of adipogenesis and osteogenesis. Our previous study showed that fad104-deficient mice died immediately after birth, suggesting fad104 to be essential for neonatal survival. However, the cause of this rapid death is unclear. Here, we demonstrate the role of fad104 in neonatal survival. Phenotypic and morphological analyses showed that fad104-deficient mice died due to cyanosis-associated lung dysplasia including atelectasis. Furthermore, immunohistochemistry revealed that FAD104 was strongly expressed in ATII cells in the developing lung. Most importantly, the ATII cells in lungs were immature, and impaired the expression of surfactant-associated proteins. Collectively, these results indicate that fad104 has an indispensable role in lung maturation, especially the maturation and differentiation of ATII cells.
Keywords
AQPSurfactant-associated proteinGATA binding proteinAlveolar epithelial type IIAlveolar epithelial type II cellsATIISrc-homology 3SH3SFTPCCSPFOXSYSECLTTFFGFEGFGAPDHKLHMEFC/EBPH&EMIGGATAaquaporinPeriodic Acid Schiffphosphate bufferLung maturationenhanced chemiluminescenceforkhead boxendoplasmic reticulumepidermal growth factorVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)fibroblast growth factorphosphatidylcholinelamellar bodyBMPmouse embryonic fibroblastsPASpolymerase chain reactionPCRCCAAT-enhancer binding proteinBone morphogenetic proteinkeyhole limpet hemocyaninglyceraldehyde-3-phosphate dehydrogenaseCRIB
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Authors
Keishi Kishimoto, Makoto Nishizuka, Takashi Ueda, Kenji Kajita, Shinya Ugawa, Shoichi Shimada, Shigehiro Osada, Masayoshi Imagawa,