Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10904847 | Experimental Cell Research | 2005 | 12 Pages |
Abstract
Although both amphiregulin and TGF-α are known to exert their effects through the EGF receptor, we find that concentrations of recombinant human amphiregulin and TGF-α that are equipotent in EGF receptor activation and mitogenesis exhibit markedly different effects on MDCK cell morphology. Amphiregulin induces a spindle-like morphology that is associated with a redistribution of E-cadherin from a Triton-insoluble to Triton-soluble pool. TGF-α does not affect epithelial morphology nor does it affect the distribution of the Triton-soluble or -insoluble pool of E-cadherin. The effects of amphiregulin on E-cadherin are associated with actin rearrangement. The morphological and biochemical effects of amphiregulin are prevented by EGF receptor blockade but require Src-family kinase activity and MAPK signaling. These results identify an action of amphiregulin that is distinct from TGF-α that may contribute to amphiregulin's participation in the pathogenesis of inflammatory disorders like psoriasis and cancer.
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Authors
Eunkyung Chung, Ramona Graves-Deal, Jeffrey L. Franklin, Robert J. Coffey,