Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10908650 | Leukemia Research | 2015 | 4 Pages |
Abstract
We assessed lineage involvement by NUP98 translocations in myelodysplastic syndromes (MDS), acute myeloid leukaemia (AML), and T-cell acute lymphoblastic leukaemia (T-ALL). Single cell analysis by FICTION (Fluorescence Immunophenotype and Interphase Cytogenetics as a Tool for Investigation of Neoplasms) showed that, despite diverse partners, i.e. NSD1, DDX10, RAP1GDS1, and LNP1, NUP98 translocations always affected a CD34+/CD133+ hematopoietic precursor. Interestingly the abnormal clone included myelomonocytes, erythroid cells, B- and T- lymphocytes in MDS/AML and only CD7+/CD3+ cells in T-ALL. The NUP98-RAP1GDS1 affected different hematopoietic lineages in AML and T-ALL. Additional specific genomic events, were identified, namely FLT3 and CEBPA mutations in MDS/AML, and NOTCH1 mutations and MYB duplication in T-ALL.
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Authors
Barbara Crescenzi, Valeria Nofrini, Gianluca Barba, Caterina Matteucci, Danika Di Giacomo, Paolo Gorello, Berna Beverloo, Antonella Vitale, Iwona Wlodarska, Peter Vandenberghe, Roberta La Starza, Cristina Mecucci,