Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10909533 | Leukemia Research | 2005 | 9 Pages |
Abstract
DNA vaccine against M-CSFRJ6-1 (macrophage colony-stimulating factor receptor cloned from the J6-1 leukemic cell line) has shown both protective and therapeutic effects. In this study, to explore the adjuvant effects of LL-37 to M-CSFRJ6-1 DNA vaccines, we constructed genetically fused vaccines encoding M-CSFRJ6-1 and LL-37(pF). After immunizing BALB/c mice, specific humoral and cellular immune responses were detected. Compared with pR (encoding the extracellular region of M-CSFRJ6-1), pF was more effective in inducing humoral and cytotoxic immune response, prolonging survival of mice challenged with SP2/0-CSFRJ6-1 tumor cells, and inducing IFN-γ and IL-4 release by splenocytes. In this study, we also constructed pLL37 (encoding the mature LL-37) and coadministrated pLL37 and pR to see whether the genetic fusion was necessary. We found that compared with pR alone, pLL37 + pR could not prolong survival of mice challenged with SP2/0-CSFRJ6-1 tumor cells. Our results suggest that when genetically fused with M-CSFRJ6-1, LL-37 could enhance adaptive immune response against M-CSFRJ6-1 in a murine model challenged with tumor cells bearing M-CSFRJ6-1.
Keywords
AMLPTKCTLPVDFAPCsTLR-4LL-37FCShCAP-18E:T ratioDCsBSAbovine serum albuminantigen-presenting cellsadaptive immunityinterferonIFNinterleukinpolyvinylidene difluoridenormal salinefetal calf serumDendritic cellsAntitumor activitiescytotoxic T lymphocyteacute myeloid leukemiaavidin–biotin–peroxidase complexPCRpolymerase chain reactionsProtein tyrosine kinaseToll-like receptor 4Macrophage colony-stimulating factor receptor
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Authors
Li-Li An, Ying-Hua Yang, Xiao-Tong Ma, Yong-Min Lin, Ge Li, Yu-Hua Song, Ke-Fu Wu,