| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10909570 | Leukemia Research | 2005 | 6 Pages |
Abstract
We directed the human natural killer (NK) cell line YT by gene transfer of a humanized chimeric immunoglobulin T cell receptor to CD33, a marker on myeloid leukemias. The chimeric receptor was generated using a CD33 specific single-chain Fv (scFv) fragment based on the humanized antibody HuM195, the human IgG1 Fc domains and the human CD3 ζ signal chain. YT cells transfected by electroporation with the chimeric receptor gene specifically lysed the acute myeloid leukemia (AML) cell line KG1. This gene-modified NK cell line available in unlimited source could be an attractive tool in immunotherapy.
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Authors
Thomas Schirrmann, Gabriele Pecher,