Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10909648 | Leukemia Research | 2005 | 8 Pages |
Abstract
This study compares the differentiation potential of mitochondrial gene knockout (Ï0) and parental HL60 cells in response to 1.25% dimethylsulfoxide (DMSO) and 10Â nM phorbol myristate acetate (PMA). Compared to HL60 cells, undifferentiated HL60Ï0 cells showed partial monocyte/macrophage differentiation, with increased CD11c and CD14 expression, decreased CD71 expression, and weak non-specific esterase staining. Differentiation along the monocyte/macrophage pathway (PMA) was more pronounced in HL60Ï0 than parental HL60 cells with increased CD11c and CD14 expression and stronger non-specific esterase staining. DMSO-exposure resulted in a poorly differentiated nuclear morphology, small respiratory burst and marginal up-regulation of CD15 expression in HL60Ï0 cells.
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Authors
Patries M. Herst, D. Matt Levine, Michael V. Berridge,