Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10909816 | Leukemia Research | 2005 | 15 Pages |
Abstract
We have identified a novel gene, upstream of the cytokine gene cluster region in 5q23-31, residing within one of the most common deleted segments associated with MDS. The novel gene exhibits significant alternative splicing generating at least six splice variants encoding four putative proline-rich protein isoforms, one of which is Golgi-associated. The gene is ubiquitously expressed and conserved among species with the C. elegans homologue being the most interesting, since it resides within an operon with two other genes, phospholipase D and dishevelled, a member of the Wnt pathway, suggesting a functional association. In addition, the novel gene and other key regulatory genes of the region, such IL3, Ril, AF5q31 and TCF-1, were found to be deleted in an atypical CML case, thus underscoring the significance of this subregion in the leukemogenesis process.
Keywords
IRF1UTRTCF-1YACWnt proteinsNIXIL3DUFSH3MTCP1 artificial chromosomeGM-CSFGFPCMLPACMDSC-terminuscDNABACN-terminusAmino-terminusribonucleic acidRNAsplice variantsInterleukin 3rapid amplification of cDNA endsdomain of unknown functionSrc homology domain 3Myelodysplastic syndromesInterferon regulatory factor 1Fluorescence in situ hybridisationChronic myeloid leukemiaFishgranulocyte macrophage-colony stimulating factorRaceWnt signaling pathwayuntranslated regionpolymerase chain reactionPCRgreen fluorescent proteincarboxy-terminusbacterial artificial chromosomeYeast artificial chromosome
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Authors
Smaragda Kamakari, Anastasia Roussou, Andrew Jefferson, Ioannis Ragoussis, Nicholas P. Anagnou,