Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10913894 | Matrix Biology | 2009 | 7 Pages |
Abstract
Although murine systems are often used to model human diseases, mice have only distant homologues of human MMP-1. Therefore, we used a technique for the targeted insertion of a single copy of a gene at the HPRT locus to compare expression of the 1G and 2G alleles. We generated transgenic mice with â 4372 bp of the human MMP-1 promoter containing either the 1G or 2G SNP in front of the lac Z (E.coli Ã-galactosidase) gene. We measured the relative expression of the transgenes in vitro in embryonic stem (ES) cells and in fibroblasts derived from embryonic mice. Our data show modest constitutive expression of Ã-galactosidase mRNA and protein from these alleles, with the 2G allele more transcriptionally active than the 1G allele. We conclude that these mice represent a model for integration of a single copy of the human MMP-1 promoter into the murine genome, and could be used to study MMP-1 gene expression in a murine system.
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Authors
Charles I. Coon, Steven Fiering, Justin Gaudet, Colby A. Wyatt, Constance E. Brinckerhoff,