Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10914771 | Molecular Oncology | 2013 | 11 Pages |
Abstract
Analysing the PIK3CA genotype of CTCs has clinical relevance with respect to drug resistance, e.g. against HER2-targeted therapy. The herein described approach including SNaPshot technology provides a simple method to characterize hotspot mutations within CTCs enriched from peripheral blood and can be easily adopted for analysing further therapeutically relevant SNPs.
Keywords
PI3Kfluorescein-isothiocyanatePIK3CA mutationsDTCsPIK3CAWGAAPCDAPIFITCHER24,6-diamidino-2-phenylindolePhosphatidylinositol-4,5-bisphosphate 3-kinaseallophycocyaninwhole genome amplificationCirculating tumor cellsMetastatic breast cancerdisseminated tumor cellsphosphatase and tensin homologphosphatidylinositol-3-kinaseSingle nucleotide polymorphismSNPPtenEstrogen receptorHuman epidermal growth factor receptor 2Progesterone receptor
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Authors
Helen Schneck, Christina Blassl, Franziska Meier-Stiegen, Rui Pedro Neves, Wolfgang Janni, Tanja Fehm, Hans Neubauer,