Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10914832 | Molecular Oncology | 2015 | 10 Pages |
Abstract
PhosphoChk1 and H2AX are useful biomarkers for ATR inhibition using a variety of immuno-detection methods, but timing may be critical. Importantly, ATR activity is detectable in non-cycling PBMCs allowing them to be used as a surrogate tissue for biomarker measurement. In PBMCs from whole blood treated with 4NQO phosphoH2AX was the most useful biomarker of ATR activity and a clinically viable pharmacodynamic assay for ATR inhibitors has been developed.
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Authors
Tao Chen, Fiona K. Middleton, Susanna Falcon, Philip M. Reaper, John R. Pollard, Nicola J. Curtin,