Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10915003 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2012 | 7 Pages |
Abstract
The induction of an adaptive response (AR) was examined in human peripheral blood lymphocytes exposed to non-ionizing radiofrequency fields (RF). Cells from nine healthy human volunteers were stimulated for 24Â h with phytohaemagglutinin and then exposed for 20Â h to an adaptive dose (AD) of a 1950Â MHz RF UMTS (universal mobile telecommunication system) signal used for mobile communications, at different specific absorption rates (SAR) of 1.25, 0.6, 0.3, and 0.15Â W/kg. This was followed by treatment of the cells at 48Â h with a challenge dose (CD) of 100Â ng/ml mitomycin C (MMC). Lymphocytes were collected at the end of the 72Â h total culture period. The cytokinesis-block method was used to record the frequency of micronuclei (MN) as genotoxicity end-point. When lymphocytes from six donors were pre-exposed to RF at 0.3Â W/kg SAR and then treated with MMC, these cells showed a significant reduction in the frequency of MN, compared with the cells treated with MMC alone; this result is indicative of induction of AR. The results from our earlier study indicated that lymphocytes that were stimulated for 24Â h, exposed for 20Â h to a 900Â MHz RF GSM (global system for mobile communication) signal at 1.25Â W/kg SAR and then treated with 100Â ng/ml MMC, also exhibited AR. These overall data suggest that the induction of AR depends on RF frequency, type of the signal and SAR. Further characterization of RF-induced AR is in progress.
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Authors
Olga Zeni, Anna Sannino, Stefania Romeo, Rita Massa, Maurizio Sarti, Abishek B. Reddy, Thomas J. Prihoda, Vijayalaxmi Vijayalaxmi, Maria Rosaria Scarfì,