Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10916914 | Pathology - Research and Practice | 2005 | 11 Pages |
Abstract
We assayed the effects of phenol red (pr), estrogen (Es), and progesterone (Pg) in three-dimensional organotypic cultures of human uterine endocervix. Small intact fragments deposited on sponges submerged in DMEM with 10% fetal bovine serum were cultured in three different conditions: with pr (DMEMpr+), without pr (DMEMprâ), and without pr but with the addition of physiological concentrations of Es and Pg [DMEMprâ(Es+Pg)]. Cell viability and cellular responses were assayed after 4, 10, and 21 days using immunohistochemistry for the expression and distribution of the following markers: mucins and mucopolysaccharides (PAS staining), pan-cytokeratins (AE1/AE3), CK19, p63, Ki-67, vimentin, estrogen receptor-α (ER-α), and progesterone receptor (PR). The fragments cultivated in DMEMpr+ showed a cuboidal, poorly differentiated epithelial phenotype and signs of stroma degeneration. In DMEMprâ, both tissue architecture and cellular heterogeneity were much better preserved: epithelial cells showed a more columnar shape, and stroma was very well conserved, maintaining cell density. The addition of Es and Pg further improved the histology and physiology of the fragments: in DMEMprâ(Es+Pg), epithelial cells retained a columnar shape, secreted mucins, and formed areas of squamous hyperplasia.
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Authors
Monica Michelini, Alfredo Rosellini, Vaclav Mandys, Tommaso Simoncini, Roberto P. Revoltella,