Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10919326 | Radiotherapy and Oncology | 2013 | 8 Pages |
Abstract
The occurrence of DNA double-strand breaks (DSBs) induced by ionizing radiation has been extensively studied by biochemical or cell imaging techniques. Cell imaging development relies on technical advances as well as our knowledge of the cell DNA damage response (DDR) process. The DDR involves a complex network of proteins that initiate and coordinate DNA damage signaling and repair activities. As some DDR proteins assemble at DSBs in an established spatio-temporal pattern, visible nuclear foci are produced. In addition, post-translational modifications are important for the signaling and the recruitment of specific partners at damaged chromatin foci. We briefly review here the most widely used methods to study DSBs. We also discuss the development of indirect methods, using reporter expression or intra-nuclear antibodies, to follow the production of DSBs in real time and in living cells.
Keywords
RadiosensitivityMre11–Rad50–Nbs1 complexPFGEDNA-PKcsIRIFSSBssDNANCOMRNPARPNHEJPI3KDSBDDRSingle-stranded DNADNA damageLETGamma raysPulse field gel electrophoresisLinear Energy TransferBiomarkerionizing radiationDNA repairRadiation dosagedouble-strand breakDNA double-strand breakDNA single-strand breaknon-homologous end-joiningphosphoinositide 3-kinaseHomologous recombinationDNA damage responsebase-pairRadiationpoly ADP ribose polymerase
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Authors
Julien Vignard, Gladys Mirey, Bernard Salles,