Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927163 | Cellular Immunology | 2005 | 8 Pages |
Abstract
PD-1 is an immunoregulatory receptor expressed on the surface of activated T cells, B cells, and monocytes. We describe four alternatively spliced PD-1 mRNA transcripts (PD-1Îex2, PD-1Îex3, PD-1Îex2,3, and PD-1Îex2,3,4) in addition to the full length isoform. PD-1Îex2 and PD-1Îex3 are generated by alternative splicing where exon 2 (extracellular IgV-like domain) and exon 3 (transmembrane domain) respectively are spliced out. PD-1Îex3 is therefore likely to encode a soluble form of PD-1. PD-1Îex2,3 lacks exon 2 and 3. These three variants have unaffected open reading frames. PD-1Îex2,3,4 lacks exon 2, 3, and 4 (intracellular domain) and contains a premature stop codon in exon 5. Activation of human PBMCs with anti-CD3Â +Â anti-CD28 monoclonal antibodies induces an increased level of each PD-1 transcript. A parallel increase in the expression of PD-1Îex3 and flPD-1 upon activation suggests an important interplay between the putative soluble PD-1 and flPD-1 possibly involved in maintenance of peripheral self-tolerance and prevention of autoimmunity.
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Authors
Christian Nielsen, Line Ohm-Laursen, Torben Barington, Steffen Husby, Søren T. Lillevang,