Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927182 | Cellular Immunology | 2005 | 10 Pages |
Abstract
Subcutaneous implantation of polyvinyl sponges represents a suitable model for studying the mechanisms of acute and chronic inflammation, granulomatous foreign-body reaction, as well as wound healing. Using such a model in rats, we studied the phenotypic and functional characteristics of dendritic cells (DC). DC were purified from the sponge exudate using a combination of separation gradients, adherence to plastics, and immunomagnetic sorting. We have shown that the number of DC progressively increased in the sponges, reaching maximal values at day 10 after implantation, followed by their decrease thereafter. Inflammatory DC expressed MHC class II molecules and myeloid markers CD11b, CD11c, and CD68. A subset of DC expressed CD4, R-MC46, DEC-205, R-MC17, and CCR1. Compared to DC isolated in the early phase of inflammation (day 6 DC), DC in the late stage of inflammation (day 14 DC) had a lower capability to stimulate the proliferation of allogeneic lymphocytes and CD4+ T cells. This finding correlated with the downregulation of CD80, CD86, and CD54 expression and the increased proportion of plasmacytoid MHC class II+ His 24+ His 48+ DC. The suppression of allogeneic lymphocyte proliferation was abrogated by the treatment of DC with lipopolysaccharide. In addition, day 14 DC exerted tolerogenic capability in co-culture with allogenic CD4+ T cells. These results correlated with the increased levels of IL-10 and TGF-β in culture supernatants and the sponge exudate.
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Authors
SaÅ¡a VasilijiÄ, Danijel SaviÄ, SaÅ¡a Vasilev, Dragana VuÄeviÄ, Sonja GaÅ¡iÄ, Ivana MajstoroviÄ, SrÄa JankoviÄ, Miodrag ÄoliÄ,