Adaptation to replating of dendritic cells synergizes with Toll-like receptor stimuli and enhances the pro-inflammatory cytokine profile

Introduction of a resting step in the DC maturation method, which is cheap and easy to implement, will improve the generation of pro-inflammatory DCs for cancer immunotherapy. These DCs enhanced Th1 polarization and IFN-γ production by CD8 T cells, both important hallmarks for the induction of efficient anti-cancer immunity.