Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10930384 | Cytotherapy | 2016 | 11 Pages |
Abstract
Background aims.âUmbilical cord (UC) has been proposed as a source of mesenchymal stromal cells (MSCs) for use in experimental cell-based therapies provided that its collection does not raise any risk to the donor, and, similar to bone marrow and lipoaspirates, UC-MSCs are multipotent cells with immuno-modulative properties. However, some of the challenges that make a broader use of UC-MSCs difficult include the limited availability of fresh starting tissue, time-consuming processing for successful derivation of cell lines, and the lack of information on identity, potency and genetic stability in extensively expanded UC-MSCs, which are necessary for banking relevant cell numbers for preclinical and clinical studies. Methods.âFactors affecting the success of the derivation process (namely, time elapsed from birth to processing and weight of fragments), and methods for establishing a two-tiered system of Master Cell Bank and Working Cell Bank of UC-MSCs were analyzed. Results.âEfficient derivation of UC-MSCs was achieved by using UC fragments larger than 7âg that were processed within 80âh from birth. Cells maintained their immunophenotype (being highly positive for CD105, CD90 and CD73 markers), multi-potentiality and immuno-modulative properties beyond 40 cumulative population doublings. No genetic abnormalities were found, as determined by G-banding karyotype, human telomerase reverse transcriptase activity was undetectable and no toxicity was observed in vivo after intravenous administration of UC-MSCs in athymic rats. Discussion.âThis works demonstrates the feasibility of the derivation and large-scale expansion of UC-MSCs from small and relatively old fragments of UC typically discarded from public cord blood banking programs.
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Authors
Irene Oliver-Vila, Maria Isabel Coca, Marta Grau-Vorster, Noèlia Pujals-Fonts, Marta Caminal, Alba Casamayor-Genescà , Isabel Ortega, Laura Reales, Arnau Pla, Margarita Blanco, Joan GarcÃa, Joaquim Vives,