| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10930466 | Cytotherapy | 2015 | 15 Pages |
Abstract
BMSC characteristics change as confluence increases. 100% confluent BMSCs may have compromised pro-angiogenesis properties but may retain their immunomodulatory properties. Supernatant lactate and glucose levels can be used to estimate confluence and ensure consistency in passage and harvest timing. Flow cytometry or microRNA expression can be used to confirm that the BMSCs have been harvested at the appropriate confluence.
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Authors
Jiaqiang Ren, Huan Wang, Katherine Tran, Sara Civini, Ping Jin, Luciano Castiello, Ji Feng, Sergei A. Kuznetsov, Pamela G. Robey, Marianna Sabatino, David F. Stroncek,