| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10930565 | Cytotherapy | 2014 | 13 Pages |
Abstract
We generated a clinically relevant number of CAR T cells from 10 mL of blood through the use of piggyBac-transposons, a 4D-Nulcleofector, and serum/xeno/tumor cell/virus-free culture system. CAR T cells exhibited marked cytotoxicity against Ph+ALL regardless of T315I mutation. PiggyBac-mediated CD19-specific T-cell therapy may provide an effective, inexpensive and safe option for drug-resistant Ph+ALL.
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Authors
Shoji Saito, Yozo Nakazawa, Akane Sueki, Kazuyuki Matsuda, Miyuki Tanaka, Ryu Yanagisawa, Yasuhiro Maeda, Yuko Sato, Seiichi Okabe, Takeshi Inukai, Kanji Sugita, Matthew H. Wilson, Cliona M. Rooney, Kenichi Koike,