| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10932563 | Developmental Biology | 2011 | 11 Pages |
Abstract
⺠Widely used 14X UAS is transcriptionally silenced in transgenic zebrafish. ⺠CpG methylation at the 14X UAS causes silencing of transgene expression. ⺠Transgenes integrated near transposons are rapidly silenced in the first generation. ⺠New, less repetitive 4X UAS reduces transgene silencing and methylation. ⺠High reporter expression persists for at least three generations using new 4X UAS.
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Authors
Courtney M. Akitake, Michelle Macurak, Marnie E. Halpern, Mary G. Goll,