Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10933714 | Developmental Biology | 2008 | 21 Pages |
Abstract
The generation of a required series of localized Ca2+ transients during cytokinesis in zebrafish embryos suggests that Ca2+ plays a necessary role in regulating this process. Here, we report that cortical actin remodeling, characterized by the reorganization of the contractile band and the formation during furrow deepening of pericleavage F-actin enrichments (PAEs), requires a localized increase in intracellular Ca2+, which is released from IP3-sensitive stores. We demonstrate that VAMP-2 vesicle fusion at the deepening furrow also requires Ca2+ released via IP3 receptors, as well as the presence of PAEs and the action of calpains. Finally, by expressing a dominant-negative form of the kinesin-like protein, kif23, we demonstrate that its recruitment to the furrow region is required for VAMP-2 vesicle transport; and via FRAP analysis, that kif23 localization is also Ca2+-dependent. Collectively, our data demonstrate that a localized increase in intracellular Ca2+ is involved in regulating several key events during furrow deepening and subsequent apposition.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Wai Ming Li, Sarah E. Webb, Ching Man Chan, Andrew L. Miller,