Quantitative proteomics and metabolomics approaches to demonstrate N-acetyl-d-glucosamine inducible amino acid deprivation response as morphological switch in Candida albicans

► We studied GlcNAc induced morphogenesis in C. albicans by 2-DE and metabolomics approaches. ► Intracellular amino acid pool goes down and amino acid biosynthesis proteins are upregulated. ► GCN4 and GCN2 genes involved in GCN response are activated in presence of GlcNAc. ► Inactivation of these genes blocks the GlcNAc induced morphogenesis. ► Amino acid starvation is the morphological switch in presence of GlcNAc.