Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10939655 | Fungal Genetics and Biology | 2005 | 12 Pages |
Abstract
The ACE1 avirulence gene allele from the rice blast fungus Magnaporthe grisea was characterized in virulent isolate 2/0/3, revealing the insertion of a 1.9Â kb MINE retrotransposon in the last ACE1 exon. MINE is a novel chimeric element composed of a transcribed non-coding sequence of 1.1Â kb (WEIRD) fused to a 5â²-truncated MGL retrotransposon. MINEs were found in high copy number in M. grisea isolates from rice (68 copies) and as a single copy in isolate CD156 from Eleusine. MINEs vary in size (1.3-6.7Â kb) with conserved 5â² WEIRD sequences and variable 3â² MGL sequences. MGLs fused to WEIRDs correspond to different 5â²-truncated MGLs with conserved 3â² ends. The organization and diversity of MINEs suggest that these retrotransposons result from independent fusions between WEIRD and 5â²-truncated MGLs. Such chimera could be formed during MGL reverse transcription as proposed for human U6-LINE1 chimeric retrotransposons and integrated into M. grisea genome using MGL machinery.
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Authors
Isabelle Fudal, Heidi U. Böhnert, Didier Tharreau, Marc-Henri Lebrun,