Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10939700 | Fungal Genetics and Biology | 2005 | 11 Pages |
Abstract
Candida albicans genes involved in mating have been identified previously by homology to Saccharomyces cerevisiae mating pathway components. The C. albicans genome encodes CaSte2p, a homolog of the S. cerevisiae α-mating pheromone receptor Ste2p, and two potential pheromones, α-F13 (GFRLTNFGYFEPG) and α-F14 (GFRLTNFGYFEPGK). The response of several C. albicans strains to the synthesized peptides was determined. The α-F13 was degraded by a C. albicans MTLa strain but not by S. cerevisiae MATa cells. The CaSTE2 gene was cloned and expressed in a ste2-deleted strain of S. cerevisiae. Growth arrest and β-galactosidase activity induced from a FUS1-lacZ reporter construct increased in a dose-dependent manner upon exposure of transgenic S. cerevisiae to α-F13. Mating between the strain expressing CaSTE2 and an opposite mating type was mediated by α-F13 and not by the S. cerevisiae α-factor. The results indicated that CaSte2p effectively coupled to the S. cerevisiae signal transduction pathway. Functional expression of CaSte2p in S. cerevisiae provides a well-defined system for studying the biochemistry and molecular biology of the C. albicans pheromone and its receptor.
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Authors
Agnieszka M. Janiak, Hasmik Sargsyan, Joe Russo, Fred Naider, Melinda Hauser, Jeffrey M. Becker,