Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10940795 | Immunobiology | 2016 | 8 Pages |
Abstract
(PVR), the receptor of TIGIT, was dramatically upregulated on the surface of mouse peritoneal macrophages when exposed to LPS. TIGIT-Fc fusion protein not only inhibited the macrophage activation, but also skewed M1/M2 balance toward an anti-inflammatory profile, especially enhanced the secretion of IL-10. The activation of TIGIT/PVR pathway in macrophages correlated with increased nuclear translocation of c-Maf, which promotes IL-10 transcription. Treatment with fibroblasts stably secreting TIGIT-Fc fusion protein significantly reversed the lethal and sublethal endotoxic shock, which facilitated peritoneal macrophages to switch towards anti-inflammatory M2 cytokine profiles. These findings highlight a novel role of the TIGIT/PVR pathway in macrophage M2 polarization and suggest that TIGIT may have the potential to optimize the treatment of macrophage-involved inflammatory diseases.
Keywords
GAPDHARG1TLRIFN-γCcl11GM-CSFCXCL1MCP-1TregFGL2eGFPPVDFMIP-1αgranulocyte-macrophage colony stimulating factorLPSTIGITMIP-1βTGF-βc-Mafarginase 1enzyme-linked immunosorbant assayAktinterleukinTransforming growth factor βELISAtumor necrosis factor-αTeffToll-like receptorpolyvinylidene difluorideDendritic cellsEffector T cellsRegulatory T cellsPVRGranulocyte colony stimulating factorgranulocyte-macrophage colony-stimulating factorG-CSFTNF-αfibrinogen-like protein 2polarizationlipopolysaccharideMacrophagemacrophage inflammatory protein-1βRANTESMacrophage inflammatory protein-1αmonocyte chemoattractant protein-1enhanced green fluorescent proteinInterferon gammaglyceraldehyde-3-phosphate dehydrogenasepoliovirus receptor
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Authors
Xi Chen, Pu-Han Lu, Lei Liu, Ze-Min Fang, Wu Duan, Zhe-Long Liu, Cong-Yi Wang, Ping Zhou, Xue-Feng Yu, Wen-Tao He,