Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10941018 | Immunobiology | 2013 | 7 Pages |
Abstract
We further assessed the biological consequence of the ficolin-A recognition of acapsular C. neoformans by investigating their interaction with lung epithelial cells (type II pneumocytes cell line A549). Flow cytometric analysis demonstrated that ficolin-A opsonized acapsular C. neoformans showed significantly increased adherence to A549 cells when exposed to acidic conditions compared to the unopsonized controls (p = 0.04). We conclude that ficolin-A binds acapsular C. neoformans via their carbohydrate recognizing fibrinogen-like domains leading to enhanced uptake by lung epithelial cells in vitro.
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Authors
Silke Schelenz, Nicole Kirchhof, Stefan Bidula, Russell Wallis, Darren W. Sexton,